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PBS (177 mM phosphate, 85 mM NaCl, 0.05% (v/v) Tween 20) at pH 5.5 was used as dilution and running buffer, while PBS (195 mM phosphate, 85 mM NaCl, 0.05% (v/v) Tween 20) at pH 7.4 was used as regeneration buffer

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State Medicaid programs that do cover Wegovy typically have more restrictive prior authorization than commercial plans: required trials of metformin (if prediabetic), required trials of two other weight-loss medications, required 6-month lifestyle intervention with documented weight logs, and BMI rechecks every 3 months with discontinuation if weight loss is under 5% at 6 months