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Briefly, N-terminal His 6 -tagged ECD was expressed in Escherichia coli inclusion bodies, isolated as inclusion body protein, solubilized in guanidine-HCl and dithiothreitol, dialyzed against guanidine-HCl to remove the dithiothreitol, and refolded using l-Arg and a 1:5 molar ratio of reduced and oxidized glutathione

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The version without DAC (also sold as Mod GRF 1-29) has a half-life of about 30 minutes and produces a more physiologic GH pulse