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Temporary effects may include mild nausea, digestive changes, or discomfort at the injection site

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Jackson Immunoresearch, 1:400) in PTwH (100 PBS 10×, 2 ml Tween 20, 1 ml of 10 mg ml −1 heparin stock solution) and 3% donkey serum at 37 °C for 7 days, followed by washes in PTwH (100 PBS 10×, 2 ml Tween 20, 1 ml of 10 mg ml −1 heparin stock solution): 1× 10 min, 1× 20 min, 1× 30 min, 1× 1 h, 1× 2 h and 1× 3 days