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β-arrestin 2 recruitment HEK293T cells (3.2 × 10 6 cells/10 cm plate) were grown for 24 h before transiently transfection with 15 μg plasmid containing GIPR, GLP-1R or GCGR tagged with Rluc8 and β-arrestin 2 with a Venus-tag at the N terminus at a ratio of 1:9 by Lipofectamine 3000 transfection reagent
copper peptide
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