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Linear calibration curves for the method ranged between 2.5–30 ng/mL, with a limit of detection of 1.7 ng/mL and a limit of quantification of 5.7 ng/mL (LLOQ)
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Moreover, the lack of interaction with key residues, such as W297 and R380 involved in agonist potency hampers the global effectiveness of neuromedin C as a full GLP1R agonist