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Overall, anagliptin was revealed to have three interactions with DPP-4 (i) Cyano group forms π-interaction at S 1 subsite with Tyr547 (proton donor), (ii) Second interaction seen to be at S 2 subsite with secondary amines forming salt bridges with Glu205 and Glu206 residues (iii) carbonyl group present at pyrazolopyrimidine moiety underwent forming H-bond interaction with Tyr547 indicating 3rd subsite is S 2 extensive
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